ABSTRACT
As a micro-wound and target-aimed technology without special limitation, Electric Pulses have been widely researched in tumor treatment and the effects have been demonstrated by a series of experiments, yet the mechanism has not been explained clearly. In this experiment, energy controllable steep pulse (ECSP) was used to treat nude mice bearing human ovarian tumor, and the result was compared with that of the control group. The expression of an important coagulant factor-tissue factor (TF) was analyzed, as TF was also a tumor indicator of invasion and metastasis, the result may indicate the relationship among ECSP, thrombosis and tumor invasion. In this study, to shed light on the mechanism of tumor treatment in electrical fields, nude mice bearing ovarian tumors were randomly divided into the treated group and the untreated group. We treated the former group and took out the tumor instantly. The thrombosis and necrosis of ovarian tumor were observed under microscope. The expression of TF was analyzed by SP immunohistochemistry and RT-PCR. Lower level of TF expression was noticed in the tumor tissue treated by ECSP, and more apparent thrombosis was also seen in this group. The results make it clear that ECSP can accelerate thrombosis and consume coagulant factors such as TF, and that low expression of TF in tumor tissue can cut out the signal paths of tumor invasion. So it is suggested that ECSP may restrain tumor invasion and metastasis by modulating thrombosis.
Subject(s)
Animals , Female , Humans , Mice , Electric Stimulation Therapy , Methods , Electromagnetic Fields , Electroporation , Methods , Mice, Nude , Neoplasm Transplantation , Ovarian Neoplasms , Metabolism , Therapeutics , RNA, Messenger , Genetics , Random Allocation , Thromboplastin , GeneticsABSTRACT
Objective:To investigate the effect of RNA interference targeting mdrl gene with ADM on ovarian cancer resistant strain SKOV 3/ADM.Methods:The expression of mdrlmRNA after transfected pshRNA-mdrl was detected by RT-PCR;MTT test and clone forming test were applied to measure the inhibition combined with adriamycin on proliferation of SKOV 3/ADM strain.Results:These pshRNA-mdrls inhibited the expression of mdrlmRNA and proliferation of SKOV 3/ADM obviously when combined with adriamycin.Conclusion:The short hairpin RNA recombinant plasmid targeting mdrl gene combined with adriamycin inhibits the proliferation of SKOV 3/ADM and increases the sensitivity to chemotherapy.